Commonly referred to as Western blot because of the similarity to the procedures described above, is used to detect antibodies to specific epitopes of electrophoretically separated subspecies of antigens. Electrophoresis of antigenic material yields separation of the antigenic components by molecular weight. Blotting of the separated antigen to nit...
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(im´u-no-blot″) to transfer proteins onto a membrane or other immobilizing matrix, analyzing or identifying them via antigen-antibody specific reactions, or the blot resulting from this process, as in a Western blot or dot blot. The term is sometimes used as a synonym of Western blot.
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Type: Term Pronunciation: im′yū-nō-blot′, im′yū-nō-blot′ing Definitions: 1. Process by which antigens can be separated by electrophoresis and allowed to adhere to nitrocellulose sheets where they bind nonspecifically and then are subsequently identified by staining with appropriately labeled antibodies.
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No exact match found.
