Photobleaching is the photochemical destruction of a dye or a fluorophore. In microscopy, photobleaching may complicate the observation of fluorescent molecules, since they will eventually be destroyed by the light exposure necessary to stimulate them into fluorescing. This is especially problematic in time-lapse microscopy. However, photobleachin...
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<biochemistry> Light induced change in a chromophore, resulting in the loss of its absorption of light of a particular wavelength. A problem in fluorescence microscopy where prolonged illumination leads to progressive fading of the emitted light because less of the exciting wavelength is being absorbed. ... (18 Nov 1997) ...
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Light-induced change in a chromophore, resulting in the loss of its absorption of light of a particular wavelength. A problem in fluorescence microscopy where prolonged illumination leads to progressive fading of the emitted light because less of the exciting wavelength is being absorbed.
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