Cryofixation is a technique for fixation or stabilisation of biological materials as the first step in specimen preparation for electron microscopy. Typical specimens for cryofixation include small samples of plant or animal tissue, cell suspensions of microorganisms or cultured cells, suspensions of viruses or virus capsids and samples of purifie...
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<procedure> Fixation processes for microscopy carried out at low temperature to improve the quality of fixation. Often very low temperatures and fast cooling are used to prevent formation of ice crystals. Cooling rates of 10,000 degrees per minute may be used and liquid nitrogen or even liquid helium temperatures used. Especially for preparin...
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Fixation processes for microscopy carried out at low temperature to improve the quality of fixation. Often very low temperatures and fast cooling are used to prevent formation of ice crystals. Cooling rates of 10,000o per minute may be used and liquid nitrogen or even liquid helium temperatures used. Especially for preparing specimens for scanning electron microscopy. Frequently no chemical treatment is used.
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cryofixation Fixation processes for microscopy carried out at low temperature to improve the quality of fixation. Often very low temperatures and fast cooling are used to prevent formation of ice crystals. Cooling rates of 10,000 degrees per minute may be used and liquid nitrogen or even liquid helium temperatures used. Especially for preparing spe...
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