(= reverse transcriptase polymerase chain reaction; reverse transcription PCR) PCR in which the starting template is RNA, implying the need for an initial reverse transcriptase step to make a DNA template. Some thermostable polymerases have appreciable reverse transcriptase activity; however, it is more common to perform an explicit reverse transcription, inactivate the reverse transcriptase or purify the product, and proceed to a separate conventional PCR.
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Type: Abbreviation Definitions: 1. Abbreviation for reverse transcriptase polymerase chain reaction.
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RT-PCR: RT-PCR (Reverse transcriptase-polymerase chain reaction) is a highly sensitive technique for the detection and quantitation of mRNA (messenger RNA). The technique consists of two parts: 1) The synthesis of cDNA (complementary DNA) from RNA by reverse transcription (RT) and 2) The amplification of a specific cDNA by the polymerase chain reac...
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Reverse transcriptase polymerase chain reaction
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