Chromatography in which the immobile phase (bed material) has a specific biological affinity for the substance to be separated or isolated, such as the affinity of an antibody for its antigen, or an enzyme for a substrate analogue.
Affinity chromatography is a method of separating biochemical mixtures based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. ==Uses== Affinity chromatography can be used to: ==Principle== The stationary phase is typically a gel matrix, often of agarose; a linear sugar molecu...Found on http://en.wikipedia.org/wiki/Affinity_chromatography
(from the article `chromatography`) A technique exhibiting great selectivity, affinity chromatography, was first described by Pedro Cuatrecasas and his coworkers in 1968. In these ... ...known in biochemistry. Examples include enzyme-protein, antigen-antibody, and hormone-receptor binding. A structural feature of an enzyme will ... [...Found on http://www.britannica.com/eb/a-z/a/24
<investigation> A technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance. ... The mixture is passed through a column containing the ligand attached to the statio...Found on http://www.encyclo.co.uk/local/20973
chromatography based on a highly specific biologic interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Any of these substances, covalently linked to an insoluble support or immobilized in a gel, may serve as the sorbent allowing the interacting substance to be isolated from...Found on http://www.encyclo.co.uk/local/21001
The use of immobilized antibody (or antigen) to select specific antigen (or antibody) from a mixture. The purified ligand is then released by disrupting the antibody–antigen interaction, for example by changing the pH.Found on http://www.roitt.com/glossary.asp
Type: Term Definitions: 1. chromatography where the absorbent has a unique chemical affinity for a particular component of the passing solution. Synonyms: affinity columnFound on http://www.medilexicon.com/medicaldictionary.php?t=17384
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