Cloning strategy for PCR products that relies on the tendency of Taq polymerase> Taqpolymerase to add an extra dA at the 3&` end of newly synthesized DNA strands, thus leaving a single base 3&` overhang. Vectors are accordingly prepared with single base dT 3&` overhangs, allowing ligation of sticky ends.
TA cloning is a subcloning technique that avoids the use of restriction enzymes and is easier and quicker than traditional subcloning. The technique relies on the ability of adenine (A) and thymine (T) (complementary basepairs) on different DNA fragments to hybridize and, in the presence of ligase, become ligated together. PCR products are usually...Found on http://en.wikipedia.org/wiki/TA_cloning
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