Look up: rapid amplification of DNA ends

1. 
   rapid amplification of DNA ends
   (= RACE; 3&` RACE; 5&` RACE) Techniques, based on the polymerase chain reaction, for amplifying either the 5&` end (5&` RACE) or 3&` end (3&` RACE) of a cDNA molecule, given that some of the sequence in the middle is already known. The two procedures differ slightly; in the more straightforward 3&` RACE, first strand cDNA is prepared by reverse transcription with an oligo dT primer (to match the poly-A tail), from an mRNA population believed to contain the target. PCR then proceeds with a gene-specific, forward-facing primer and an oligo-dT reverse facing primer. 5&` RACE is an example of anchored PCR; the first-strand cDNA population is tailed with a known sequence, either by homopolymer tailing (eg. with dA) or by ligation of a known sequence. PCR then proceeds as before with a primer specific for the gene, and one specific for the added tail.
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2. 
   Rapid Amplification of cDNA Ends
   `RACE`, or `Rapid Amplification of cDNA Ends`, is a technique used in molecular biology to amplify the ends of messenger RNA (mRNA) transcripts using a specialized reverse transcription-polymerase chain reaction (RT-PCR). It allows the amplification of an unknown end portion of a transcript using known information from the center of the transcript. It can be used to obtain the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of mRNA. This technique i...
   Found on http://en.wikipedia.org/wiki/Rapid_Ampli